Changing the game: absolute protein quantification by relating histone mass spec signals to DNA amounts and cell numbers

One thing system biologists want is to have by and large absolute protein concentrations or copy numbers per cells available cheaply for their models leveraging all sorts of omics data. Looks like such results can now be easily delivered based on a study published on the 15th of September by the Mann lab in Molecular & Cellular… Continue reading Changing the game: absolute protein quantification by relating histone mass spec signals to DNA amounts and cell numbers

Pleasingly Parallel MCMC: cracked wide open for MapReduce and Hadoop

MCMC methods guarantee an accurate enough result (say parameter estimation for a phylogenetic tree). But they give it to you usually in the long-run and many burn-in steps might be necessary before performing ok. And if the data size grows larger, the number of operations to draw a sample grows larger too (N -> O(N)… Continue reading Pleasingly Parallel MCMC: cracked wide open for MapReduce and Hadoop

2 recent Global Alliance for Genomics and Health standard candidates: ADAM and Google Genomics

Global Alliance for Genomics and Health includes > 150 health and research organizations to progress/accelerate secure and responsible sharing of genomic and clinical data. GA4GH (for short) is something you will here about more and more in the short term future. In the context of genomics standards think of mainly data formats and code to access and process… Continue reading 2 recent Global Alliance for Genomics and Health standard candidates: ADAM and Google Genomics

3 recent Hadoop/MapReduce applications in the life sciences: RNA structure prediction, neuroimaging genetics, EEG signal analysis

3 open access papers, 3 prototypes, source code available only for 1, healthy diversification of topics. 1. Enhancement of accuracy and efficiency for RNA secondary structure prediction by sequence segmentation and MapReduce code available: haven’t found it referenced in the paper Our previous research shows that cutting long sequences into shorter chunks, predicting secondary structures of… Continue reading 3 recent Hadoop/MapReduce applications in the life sciences: RNA structure prediction, neuroimaging genetics, EEG signal analysis

Google invests into DNAnexus: aging-driven big data bioinformatics without the Hadoop Ecosystem?

First time DNAnexus made me think a little about what they can achieve was when they came up with an alternative search and browse interface for the complete Sequence Read Archive (SRA) database. They came to the ‘rescue’ as NCBI discontinued SRA in 2011 although later they’ve changed their mind, so SRA is still up and running there.… Continue reading Google invests into DNAnexus: aging-driven big data bioinformatics without the Hadoop Ecosystem?

Coming of age for proteogenomics: 10% less human protein coding genes based on mass spec proteomics data?

Guessing the number of real protein-coding genes is an ‘ancient’ bioinformatics game and now a new argument & newish research field has been applied to this problem. Proteogenomics can refer to different type of studies but the basic idea is that mass spectrometry peptide/protein evidences are used to improve genome annotations. Now a joint Spanish –… Continue reading Coming of age for proteogenomics: 10% less human protein coding genes based on mass spec proteomics data?