David Secko writes: “Today, it is thought that one third of the proteins present in a typical mammalian cell are covalently bound to phosphate (i.e. they are phosphorylated at one time or another)” Well I haven’t checked what kind of measurement the above 1/3 estimation is based on but if true it is no wonder… Continue reading How overrated is phosphorylation? – an opinion
Building and using low budget but high tech devices at home is a main motivation behind hacking. A Harvard Chemistry Research Group now created a microchannel producing device using a Hewlett Packard 7550A Graphics Plotter (see some eBay prices) to perform a diagnostic protein assay with it amongst others. /See my SciFoo microfluidics coverage./ According… Continue reading Low budget, high tech: Microfluidics device out of a $50 plotter!
A partnership between the Journal of Visualized Experiments and big science publisher Wiley-Blackwell: the JoVE guys will give the technology, the art of making video experiments and Wiley provides the established network, audience on its Current Protocols site. I wonder what will be the access status of those videos: current JoVE videos are freely available,… Continue reading New JoVE helps old Wiley to publish video articles on Current Protocols
One friend of mind is looking for a proper double staining protocol for laminin and BrdU on fixed rat striatal muscle slices. Can anybody help, please? The following antibodies are used: Primary antibodies:
Have you ever wanted to isolate subcellular components from molecules to organelles with the old but ever improving ultracentrifuge method but were unable to figure out a correct protocol as the basics were not that clear? To get an optimal protocol you have to take account the biological entity and pellet you want, the maximum… Continue reading Pelletology: the essence of ultracentrifugation in 14 slides
Genome Technology, the heavy trafficked New York based biotech website (also a printed monthly magazine) just launched the beta version of MethodShare as “a place for people to discuss methods and tools, recommend methods papers to one another” according to Ciara Curtin, senior editor. The site will be coming out of beta soon. How the… Continue reading Genome Technology launches MethodShare beta!
Let’s give a chance to audio articles, a new initiative being trialed by Nature Clinical Practice. “These are FREE full-text audio versions of printed content from the March 2007 issue of Nature Clinical Practice Rheumatology. The aim of the Nature Clinical Practice journals is ‘to translate the latest findings into clinical practice’ by highlighting important… Continue reading Nature Clinical Practice audio articles: keeping busy doctors updated