David Secko writes: “Today, it is thought that one third of the proteins present in a typical mammalian cell are covalently bound to phosphate (i.e. they are phosphorylated at one time or another)” Well I haven’t checked what kind of measurement the above 1/3 estimation is based on but if true it is no wonder… Continue reading How overrated is phosphorylation? – an opinion
Building and using low budget but high tech devices at home is a main motivation behind hacking. A Harvard Chemistry Research Group now created a microchannel producing device using a Hewlett Packard 7550A Graphics Plotter (see some eBay prices) to perform a diagnostic protein assay with it amongst others. /See my SciFoo microfluidics coverage./ According… Continue reading Low budget, high tech: Microfluidics device out of a $50 plotter!
A partnership between the Journal of Visualized Experiments and big science publisher Wiley-Blackwell: the JoVE guys will give the technology, the art of making video experiments and Wiley provides the established network, audience on its Current Protocols site. I wonder what will be the access status of those videos: current JoVE videos are freely available,… Continue reading New JoVE helps old Wiley to publish video articles on Current Protocols
One friend of mind is looking for a proper double staining protocol for laminin and BrdU on fixed rat striatal muscle slices. Can anybody help, please? The following antibodies are used: Primary antibodies:
Have you ever wanted to isolate subcellular components from molecules to organelles with the old but ever improving ultracentrifuge method but were unable to figure out a correct protocol as the basics were not that clear? To get an optimal protocol you have to take account the biological entity and pellet you want, the maximum… Continue reading Pelletology: the essence of ultracentrifugation in 14 slides
Genome Technology, the heavy trafficked New York based biotech website (also a printed monthly magazine) just launched the beta version of MethodShare as “a place for people to discuss methods and tools, recommend methods papers to one another” according to Ciara Curtin, senior editor. The site will be coming out of beta soon. How the… Continue reading Genome Technology launches MethodShare beta!
Let’s give a chance to audio articles, a new initiative being trialed by Nature Clinical Practice. “These are FREE full-text audio versions of printed content from the March 2007 issue of Nature Clinical Practice Rheumatology. The aim of the Nature Clinical Practice journals is ‘to translate the latest findings into clinical practice’ by highlighting important… Continue reading Nature Clinical Practice audio articles: keeping busy doctors updated
The first lab of Pimm’s new series turned out to be the Bernstein Laboratory at the University of California, San Francisco focusing on heart muscle regeneration. Unlike other professors, Harold Bernstein is extra fast, he answered my questions within 8 hours. This web-availability, rare within academic circles, positively correlates with the design and functionality of… Continue reading Stem Cell Lab Monitor: the Bernstein Laboratory at UCSF
The question of the blogxperiment series is: What are the good ways to summarize peer-review articles for a more general readership and transmit scholarly knowledge and literature? After showing an abstract and some graphics of a sample review on mitochondrial fusion and division apparatus here I wonder whether the simple copy of the Contents is… Continue reading Blogxperiment: science article popularization with Contents
In the last post on “supporting information” section I claimed that the problematic status of supporting information comes from the heterogeneity of its data, on the one hand genuine online multimedial files, on the other hand “paperlike” data. Big differences also occur concerning the importance of the data. The source of the heterogeneity is the… Continue reading Let’s make ‘supplementary’ peer-review scientific videos free and youtubish!
I’ve worked all day and still have some commitments with my stemy cells, you know feed ’em, split ’em, torture’ em, and voyeur ’em so this is a weekend on session. Tomorrow is the same, I am adapted to this lifestyle and my girlfriend is tolerant blissfully. Left you can see my first rat fibroblast… Continue reading These GFP cells are really green, in rats too. (weekend on)