Mason-Dixon line in the lab

Mason-Dixon line

Left side: QIAamp DNA Mini Kit for 250 DNA preps ($556.00) for purification of genomic, mitochondrial, bacterial, parasite, or viral DNA

Right side: RNeasy Mini Kit (50) for purification of up to 100 ug total RNA from animal cells or tissues, yeast, or bacteria ($227.00)

6 thoughts on “Mason-Dixon line in the lab

  1. Yeah, kits are so lame. We should all be extracting and purifying our restriction enzymes and recrystallizing our reagents out of spent culture media. We should also all be soldering our own motherboards and coding our own OSes. Who needs consistency and standards?

  2. Soft-updates guarantees that the only filesystem inconsistencies on unclean shutdown are leaked blocks and inodes. To resolve this you can run a background fsck or you can ignore it until you start to run out of space. We also could’ve written a mark and sweep garbage collector but never did. Ultimately, the bgfsck is too expensive and people did not like the uncertainty of not having run fsck. To resolve these issues, I have added a small journal to softupdates. However, I only have to journal block allocation and free, and inode link count changes since softdep guarantees the rest. My journal records are each only 32bytes which is incredibly compact compared to any other journaling solution. We still get the great concurrency and ability to ignore writes which have been canceled by new operations. But now we have recovery time that is around 2 seconds per megabyte of journal in-use. That’s 32,768 blocks allocated, files created, links added, etc. per megabyte of journal.

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