Pimm – Partial immortalization

A Biotech Geek (micro)Blogger’s adventures through science, technology and the web…

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Skills

Research Techniques/Computer Skills:

Experimental Research Techniques

• In vitro cell culture techniques
• Isolation of human cardiomyocytes, human amniotic, mouse and rat mesenchymal stem cells
• Stem cell culture maintenance: human amniotic cells, human and mouse mesenchymal cells
• Laser scanning confocal microscopy
• Polymerase Chain Reaction: mitochondrial DNA
• Single-stranded Conformation Polymorphism (SSCP)
• SDS-PAGE
• Western blot
• rho 0 cells
• Mitochondria isolation
• Immunocytochemical staining
• Flow cytometry
• Transplantation techniques on rats: local and systematic
• Cold lesion model on rat and mouse
• Rat neurological test
• Optical Waveguide Lightmode Spectroscopy (OWLS)
• Planning and building biological databases

IT

• O’Reilly School of Technology online courses, 4 Continuing Education Credits
(CEUs) from the University of Illinois: Linux/ Unix 1: file system, basics of system
administration and user management, 6 CEUs: Introduction to Object-Oriented
Programming via Java
• Basic programming: Java, Perl, Perl/CGI, MySQL, SQLite, HTML/CSS, XML, R,
Ruby, Python, C, Objective C
• Mac OS X, Keynote, iMovie, Comic Life, Safari
• MS Office (Excel, Word, PPT), IE,
• Google Docs, Google Chrome, Mozilla Firefox,
• Basic Adobe Photoshop, Feed Readers,
• Wiki software: TWiki, Kwiki, MediaWiki,
• Basic Mac/iPhone application development: Xcode, Dashcode, Interface Builder,
iPhone Simulator
• Basic Android mobile application development: Eclipse/Java/XML
• Microcontroller: Arduino Diecimila, Arduino Bluetooth
• Basic RFID: using the Parallax RFID Reader with Arduino microcontroller

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